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ATP, QAC & Spray Volume Testing

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Corona Virus Sanitation
ATP (Adenosine Triphosphate) Biological Residues Test
QAC (Quaternary Ammonium Compounds) Test

In short, ATP testing is used to test for proteins on a surface and QAC test strips are used to test the strength of the formula being applied.

ATP Testing. The ATP test is a process of measuring actively growing microorganisms through the detection of biological residue on surfaces. To verify the cleanliness of surfaces we use ATP swabs and if protein residue is found the reagent turns purple. This instantly validates surface hygiene, allowing immediate corrective action to be taken where necessary.

QAC Testing is done on-site during our bio-fogging process by placing a strip or strips (depending on the size and shape of the room being treated) of test paper at the furthest point away from our equipment. After bio-fogging the room, we check the test strips to ensure that an adequate amount of bio-solution has been applied.

Spray Volume Testing is something that is done in house to calibrate the particle size of the biocide being expelled from our bio-fogging machines (too large a particle will leave surfaces wet or feeling damp and will take longer to dry). To do this we use a 20″ x 20″ Cobalt Chloride cloth which detects the presence of moisture and accurately displays the spray pattern. It is a fast and easy way to identify our spray patterns to ensure maximum spread with minimum particle size. The Cobalt chloride cloth is responsive to 50% relative humidity and above, and any direct measure of an aqueous solution is detected. We can also use it on-site to visually test the coverage of our bio-fogging process in large rooms, as exposure to 55 percent relative humidity or greater is sufficient enough to cause a colour change.


Testing Kits Information Sheets


ATP Testing

ATP is a molecule found in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally occurring firefly enzyme luciferase using a luminometer. The amount of light produced is directly proportional to the amount of ATP present in the sample.

Surface Protein Plus Swabs are a rapid hygiene test which detects whether a surface has been cleaned effectively. These colour changing swabs provide you with the means to monitor cleaning procedures first hand, quickly and effectively on a day to day basis. The unique “swab-click-read” technology is easy to use and detects protein residue on surfaces in 10 minutes or less. The faster the swab turns purple the higher the level of contamination on a surface, but if it’s green, it’s clean! The Clean-Trace Surface Protein Plus swabs are a reliable solution to monitor biological contamination on surfaces in real-time, helping you to manage your cleaning and protect your customers. With no instrumentation required the swab is all you need.


Precision Laboratories QAC test papers and strips

The High Level QAC Test Strip (Quaternary Ammonium Compounds) measures the range of 1-1500 ppm in increments of 200, 400, 750, 1000 and 1500 ppm, and gives results in just seconds. Check for higher levels of QAC when testing stronger solutions that kill pathogenic organisms, as opposed to testing for residual QAC with our Low Level QAC test strip, or our standard QAC Quick Response Test Strip.

Precision Laboratories QAC test papers and strips utilise an effect commonly referred to as the “protein-error of indicators” to quantify the QAC ppm levels.  While first observed with proteins (thus the name), the technique utilises pH indicators and buffering systems to create an environment where the test paper or strip reacts (develops colour) proportional to the amount of analyte (QAC) present.  The PL QAC test papers and strips have colour charts calibrated to solutions prepared from OASIS 146, an industry standard QAC product.  The performance of the papers and strips with alternate QAC products should always be verified.  In addition, any additives should be evaluated to see if they contribute to the “protein-error” effect.

How to Use:

  1. Remove a High Level QAC test strip from the vial by holding it between your index finger and thumb. Do not touch the test pad with your fingers.
  2. Dip the strip into the solution to be tested and remove immediately.
  3. Compare the test strip to the included colour chart immediately. It is very important for the accuracy of the results to read the strip immediately (the maximum wait time is 5 seconds).

The Precision Laboratories QAC test strips have been calibrated for use with all the commonly used hyamine and steramine quat (quaternary ammonium chloride) solutions. Some commercially available strips have errors as high as 50%.

QAC 1500 ppm Test Strip Data Sheet


Recommended concentration of bleach for dangerous pathogens?

All available chlorine has some biocide strength, although hypochlorous acid is a far stronger biocide than hypochlorite ion or chloramines. In solutions at pH of 5-7, hypochlorous acid is the most prevalent and most sanitising solutions work best in the neutral to slightly acidic pH range. Chlorine bleach at concentrations of at least 1000ppm is an effective germicide for dangerous pathogens such as MRSA (Methicillin Resistant Staphylococcus aureus), VRE (Vancomycin Resistent Enterococci) and Clostridium difficile.